Testosterone is a sex hormone that plays a crucial role in the development and maintenance of male sexual characteristics, although it is also present in lower levels in individuals of all genders. It has various functions, including regulating muscle mass, bone density, and the production of red blood cells.

Testosterone circulates in the bloodstream primarily in two forms: free and bound to proteins. Free testosterone is considered the biologically active and readily available portion of the hormone. It can have a more direct impact on various physiological functions, including libido, muscle mass, and mood.

Measuring both free and total testosterone provides a comprehensive and nuanced assessment of an individual's hormonal status.

Who should take this test?

Testosterone levels may be tested in cisgender men, transgender men, and gender-diverse individuals assigned male at birth to assess hormonal health, monitor the effects of testosterone therapy, or address specific health issues related to testosterone.

Testosterone levels may also be tested in cisgender women, transgender women, and gender-diverse people assigned female at birth when there are medical concerns, such as polycystic ovary syndrome (PCOS) or other conditions that may lead to elevated testosterone levels.

Individuals who are undergoing hormone therapy as part of their gender-affirming care may have their testosterone levels monitored to ensure that hormone levels are within a safe and effective range.

What is included in this test?

This blood test measures:

• Total testosterone
• Free testosterone

This test uses equilibrium ultrafiltration to separate the free testosterone from the bound testosterone in the blood sample.

How will taking this test benefit me?

Early detection of hormonal imbalances can prevent the development of more severe symptoms or complications associated with these conditions. For those undergoing hormone therapy, it is a valuable tool for monitoring the effectiveness and safety of that therapy.